Enzymes of Intermediary Carbohydrate Metabolism in the Obligate Autotrophs Thiobacillus thioparus and Thiobacillus neapolitanus

Assimilation and metabolism of exogenous organic compounds by the strict autotrophs Thiobacillus thioparus and Thiobacillus neapolitanus.

The assimilation and utilization of the individual carbon atoms of pyruvate and acetate by cells of Thiobacillus thioparus and T. neapolitanus, in the presence and absence of an energy source, were studied by use of radioactive substrates. Both organisms produced (14)CO(2) from (14)C-labeled pyruvate, but more came from carbon 1 than from carbons 2 or 3. The conversion of the carbons of acetate...

Properties of phosphoribulokinase from Thiobacillus neapolitanus.

Partially purified preparations of ribulose-5-phosphate kinase (specific activity, 50 to 125 mumoles per min per mg of protein) were employed in a series of kinetic experiments in the presence of several concentrations of H(+), Mg(2+), adenosine triphosphate (ATP), and phosphoenolpyruvate (PEP). The pH optimum of the enzyme was found to be 7.9; at this pH and above, response of the enzyme to va...

Evidence for the Presence of Certain Tricarboxylic Acid Cycle Enzymes in Thiobacillus Thioparus.

Cooper, Robert C. (Michigan State University, East Lansing). Evidence for the presence of certain tricarboxylic acid cycle enzymes in Thiobacillus thioparus. J. Bacteriol. 88:624-629. 1964.-Various tricarboxylic acid cycle enzymes appear to be present in Thiobacillus thioparus. Cell-free extracts of T. thioparus were active for a number of tricarboxylic acid cycle enzymes, including aconitase, ...

Reduced nicotinamide adenine dinucleotide oxidation by Thiobacillus neapolitanus and Thiobacillus strain C.

A. J. Smith, J. London, and R. Y. Stanier (J. Bacteriol. 94:972, 1967) reported that a number of obligate autotrophs, including Thiobacillus thiooxidans and T. thioparus, lacked reduced nicotinamide adenine dinucleotide (NADH2) oxidase activity, although this activity was readily detected in the facultative autotrophs T. intermedius and Hydrogenomonas eutropha. They suggested that the absence o...

Energy conservation in Thiobacillus neapolitanus C6 sulphide and sulphite oxidation.